Fig. 8
From: Effect and mechanism of miRNA-144-5p-regulated autophagy in older adults with Sarcopenia
Detection of cell senescence and myogenic differentiation. (A) β-galactosidase staining. (B) Detection of MHC protein expression by Western Blot. (C-D) Immunofluorescence detection of the expression of MHC protein. (E-F) Western Blot detected senescence-related proteins acetylated p53, p53 and p21, the expression and ratio of autophagy marker LC3II/LC3I, and the expression of Beclin-1, p62 and Atg2A in each group. Note: Scale bar = 100 μm in Figure A, Scale bar = 50 μm in figure C. * p < 0.05, ** p < 0.01 vs. mimic-NC (ANOVA analysis followed by Tukey’s post hoc test). The error bars represent standard deviation (SD), n = 3