Fig. 5
The Zn-RS adjuvant formulation drove the expansion of GCB and memory B/T cells. Young adult (6-week-old) and aged (15-month-old) C57BL/6 mice were subcutaneously injected with 10 μg of the STFK1628x protein (formulated in the Al-001 or Zn-RS adjuvant) on days 0 and 21 post-initial treatment. A Spleen and lymph node samples were collected 14 days post-initial injection. Absolute numbers of GCB cells, plasma cells and TFH cells. B Draining inguinal LNs and spleens were harvested 14 days following the second injection. Absolute numbers of memory B cells and T cells. The data are presented as the means ± SDs (n = 6/group). Statistical significance was evaluated using Mann–Whitney U test (two-group comparison) or one-way ANOVA (multi-group comparison). *p < 0.05, **p < 0.01, ***p < 0.001