Fig. 4

The Zn-RS adjuvant formulation elicited robust APC responses. C57BL/6 mice (young adults: 6 weeks old; aged: 15 months old) received subcutaneous injections of 10 μg of the STFK1628x protein formulated with either the Al-001 or Zn-RS adjuvant following single- or double-dose immunization protocols. Spleens and draining lymph nodes were aseptically collected 7 days post-primary immunization for immune cell profiling. A Absolute numbers of macrophages, monocytes and neutrophils; B myeloid DCs and lymphocyte DCs; C B cells and T cells. The data are presented as the means ± SDs (n = 6/group). Statistical significance was evaluated via the Mann‒Whitney U test (two-group comparison) or one-way ANOVA (multi-group comparison). *p < 0.05, **p < 0.01, ***p < 0.001