Fig. 1

Engraftment of HSC-donor cells and immune cells in transplanted RAG1^−/− hosts. (a) An equal number of 600 LT-HSCs, isolated from young or old CD45.1/STEM donor mice, were transplanted into irradiated young CD45.2⁺ RAG1^−/− recipients. (b)De novo reconstitution of immune cells usually was monitored at 6 and 12 weeks post transplantation in the peripheral blood (PB) and at 18 weeks post transplantation in the spleen. (c) HSCs from young (DY-HSC) (n = 4) or old (DO-HSC) mice (n = 4) were transplanted into irradiated young CD45.2⁺ RAG1^−/− recipients and the kinetics of cell reconstitution was analyzed. The percentage of donor-derived CD45.1⁺ cells, CD19⁺ B cells, CD4⁺ T cells, CD8⁺ T cells and CD45.1⁺ myeloid cells to total white blood cells (WBCs) was determined at 6, 12 and 18 weeks post transplantation by flow cytometry. (d) HSCs from young (DY-HSC) (n = 3) or old (DO-HSC) mice (n = 4) were transplanted into irradiated young CD45.2⁺ RAG1^−/− recipients and the frequencies of donor-derived CD45.1⁺ WBCs (left panel) and its Lin(-)/Sca-1(+)/c-Kit(+) LSK cell fraction (middle panel) was analyzed in the bone marrow (BM) at 18 weeks post transplantation by flow cytometry. Furthermore, the LSK cell pool was analyzed for lympho-myeloid primed progenitors (LMPPs), short-term HSCs (ST-HSCs) and long-term HSCs (LT-HSCs) (right panel). Statistical significance between the individual cell populations was determined using the unpaired students t-test. p < 0.05*, p < 0.01** If not indicated differences were not significant. Mean values±SD are shown. Created with Biorender.com