Fig. 1

In-depth T cell subsets associated with low or high multiple vaccine response. (a) UMAPs presenting FlowSOM meta clusters for CD4+, (b) CD8 + and (c) γδ + T cells. (d) Projection of the percentage of 10 immune cell subsets that were statistically significant between low and high vaccine responders onto the two Principal Components (PC) (e) Contribution of immune cell subsets on PCs colored by their square of cosine (cos2). (f) The percentage of manually gated CD31 + true naïve CD8+, (g) CD31 + true naïve CD4+, and FlowSOM identified (h) CD8c26 (CD31 + naïve CD8 + T cells), (i) CD4c23 (CD31 + naïve CD4 + T cells), (j) CD4c2 (HLA-DR+/- CD4 + Tem Treg), (k) CD8c25 (CD38 + naïve CD8 + T cells), (l) GDc11 (Helios + CCR4 + Tcm-like γδ + T cells), (m) GDc22 (KLRG1 + CD159a + Tte-like γδ + T cells)in vaccine response groups. CD45RA + CCR7-CD28-CD57+ (Terminal effector, Tte) The significance between vaccine response groups was determined by Mann-Whitney-Wilcoxon test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001