Fig. 7
Lipidomic profiling of lung homogenates from the elderly and young mice infected or not (mock) with SARS-CoV-2. A Differentially expressed lipids (DELs) between the two mouse groups for the same infection time-point (mock, 3 DPI, 5 DPI, 7 DPI). Color blue to red indicates log2 of the fold change (FC) in the aged mice in comparison with the young mice and the dot size indicates the P value calculated with uncorrected unpaired T-test assuming equal group variance (Mean n = 3–5/group). B Levels of Lipids significantly different according to age when adjusted (marked with #) or not with the infection time-point. Lipids with an unadjusted P value below 0.05 in the bar graph. P values were calculated with a linear model based on the limma method [106] (n = 3–5/group). C Specific Lipids showed a significant interaction with age and infection time-point. Lipids that display a raw P value below 0.05 at the Two-way ANOVA tests are shown in the Venn diagram (n = 3–5/group). The 27 lipids that show no only interaction and differed only with the stage of infection were ± 19(20)-DiHDPA, ± 8-HEPE, ± 16-HDPA, ± 17(18)-DiHETE, ± 20-HDPA, Prostaglandin E3, RvD2 n−3 DPA, ± 5-HEPE, ± 7(8)-DiHDPA, ± 13-HDPA, 13(S)-HODE, ± 8-HDHA, 9(S)-HODE, 9(R)-HETE, ± 14(15)-DiHETE, 11(S)-HETE, ± 5(6)-DiHETE, Thromboxane B2, ± (18)-HEPE, 17(S)-HDHA, ± 4-HDPA, ± 7-HDPA, ± 11-HDPA, Protectin DX, 8-isoPG E2 and 5-F2t-IsoP