Fig. 5
Leucocyte recruitment in mouse lungs following SARS-CoV-2 infection. Lung sections were stained with antibodies against mouse CD4 (A), CD8a(B), NK1.1(C), F4/80 (D) or GR1(E). Stained tissues were scanned, and positive cells were quantified on the whole section. Results were expressed as number of positive cells by mm.2 of tissue section (Mean ± SD, n = 3–5/group). Images are representative of each immunostaining and shown beside each graph. For each group, infection time points were compared with an Uncorrected Fisher's LSD or Uncorrected Dunn’s test with their mock condition according to the data distribution. Time points between groups were compared with the Unpaired T-test or Mann Whitney test. Effects of the age and infection time point factors were evaluated using a mixed-effect model. P value: > 0.05 (ns), < 0.05 (*), < 0.0021 (**) and < 0.0002(***)