Fig. 4

Microglial activation was detected by using flow cytometry. (A) The gating strategy for microglia. The gate represents CD11b+CD45int-expressing microglia. (B) Flow cytometry plots showing CD86, MHC-II and CD200R expression on the surface of microglia. (C) The percentages of CD86+, MHC-II+ and CD200R+ cells among the CD11b+CD45int microglia. * P < 0.05, ** P < 0.01. FMO: fluorescence minus one control