Fig. 2
From: Elocalcitol mitigates high-fat diet-induced microglial senescence via miR-146a modulation
High-fat diet induces senescence in the hypothalamus. (A) Representative dot plots of flow cytometry showing β-gal positive CD45 + CD11b + microglial cells. (B) Quantification of β-gal positive CD45 + CD11b + microglial cells in the hypothalamus. (C-D) RT-qPCR analysis of p16 and p21 mRNA expression. Number of animals = 5–8. Student’s t-test (A, C) and Mann Whitney test (B). Data are presented as mean ± SEM; ** p < 0.01, *** p < 0.001