Fig. 5

Presence of IgM⁺ astrocytes. A Representative section of zone 2 from a 12-month-old SAMP8 mouse immunostained with anti-GFAP (green), and IgM (red) antibodies (A); note that astrocytes not close to the fissures were IgM⁻ (green-stained, empty arrowheads, A.1) while only the astrocytes closest to the fissures were IgM⁺ (yellow (green + red)-stained, full arrowheads, A.2). B Effect of the preadsorption of IgM antibodies with a 0.4 M sugar mixture in IgM⁺ astrocytes in a representative section of a 12-month-old SAMP8 mouse. C Effect of the pre-treatment with 200 U/mL of ƴ-amylase on the staining with IgM in IgM⁺ astrocytes in a representative section of a 12-month-old SAMP8 mouse. D Effect of a 40 min boiling pre-treatment on the staining with IgM in IgM⁺ astrocytes in a representative section of a 12-month-old SAMP8 mouse. Scale bars: 100 µm