Fig. 7

Melatonin enhances the expression of JAK3/p-STAT5 and T-bet in spleen NK cells of aged mice. (A) Following melatonin treatment, flow cytometry was employed to detect JAK3 expression in NK cell subgroups at various developmental stages. (B) Statistical Graph Depicting JAK3 fluorescence intensity in different subgroups of spleen NK Cells. (C) Following melatonin treatment, flow cytometry was utilized to detect the expression of p-STAT5 in NK cell subgroups at various developmental stages. (D) Statistical graph illustrating fluorescence intensity of p-STAT5 in different subgroups of splenic NK Cells. (E) Following melatonin treatment, flow cytometry was employed to detect the expression of T-bet in NK cell subgroups at various developmental stages. (F) Statistical graph depicting T-bet fluorescence intensity in different subgroups of spleen NK Cells. (G) Following melatonin treatment, flow cytometry was employed to detect the expression of Eomes in NK cell subgroups at various developmental stages. (H) Statistical graph depicting T-bet fluorescence intensity in different subgroups of spleen NK Cells. (I) Splenic NK cells were isolated from aged mice (1 × 10^6), pre-treated with BD750 (STAT5 antagonist, 1µM) for 2 h, followed by treatment with 100µM melatonin for 24 h, NK cell STAT5 expression was detected using flow cytometry. (J) The statistical graph of NK cell STAT5 expression. K. Splenic NK cells were isolated from aged mice (1 × 10^6), pre-treated with BD750 (STAT5 antagonist, 1µM) for 2 h, followed by treatment with 100µM melatonin for 24 h, NK cell T-bet expression was detected using flow cytometry. L. The statistical graph of NK cell T-bet expression. The data is presented as means ± SD. n = 6. Unpaired two-tailed Student’s t-tests or one-way ANOVA. were conducted using Prism software. A p-value of less than 0.05 was considered statistically significant. *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001