Fig. 1

Testing the Association of CD4⁺ and CD8⁺ T_EMRAs with global cognitive function. (A) Representative flow cytometry gating strategy for lymphocyte subsets. The strategy begins with gating on lymphocytes, excluding doublets and dead cells. T cells are gated on CD3⁺; CD4⁺ T cells are CD3⁺ CD4⁺; CD8⁺ T cells are CD3⁺ CD8⁺. The CD4⁺ and CD8⁺ T cells are then further divided into central memory (T_CM): CD45RA⁻CCR7⁺, naive: CD45RA⁺CCR7⁺, effector memory (T_EM): CD45RA⁻CCR7⁻, and terminally differentiated effector memory re-expressing CD45RA (T_EMRA): CD45RA⁺CCR7⁻. Association of CD4⁺ and CD8⁺ T_EMRAs with (B) CDR (C) MMSE and (D) MoCA. Circles represent individual participants. The red lines represent the mean and 95% confidence interval for the linear regression. See also, Table 2