Fig. 5

Plaques of aged male and female Ldlr^−/− mice differ in myeloid cell proportions. A UMAP plots and stacked diagram of myeloid cell clusters in the aorta of Ldlr^−/− mice. B Volcano plot displaying differentially expressed genes of the total myeloid cell cells between aged male and female Ldlr^−/− mice. C Sex-specific expression levels of Il1b⁺ M1-associated genes. D Dot plot displaying the sex-specific expression of biological process-associated genes in macrophage clusters. Sex-specific gene expression of E Trem2⁺ M2-related genes in cluster 4 and F mast cell associated-markers in cluster 9. G Pathway enrichment of myeloid cells in male and female mice. Green dots: significantly enriched pathways; red dots: interleukin-related pathways; white dots: insignificantly enriched pathways; black dots: insignificantly unenriched pathways