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Fig. 4 | Immunity & Ageing

Fig. 4

From: Terminally exhausted CD8+ T cells contribute to age-dependent severity of respiratory virus infection

Fig. 4

Aged T cells transplanted into young mice recapitulated aged CD8+ T cell phenotype. A Syngeneic transplant experimental design. Lethally irradiated CD45.2 aged mice were reconstituted with T lymphocytes from young or aged CD45.1 donors, young B cells, and Rag1−/− BM. B, C Aged mice that received young T lymphocytes (i.e. YT—> AH) had improved CD8+ tetramer+ response compared to AT—> AH control. AT—> YH had a diminished tetramer response compared to YT—> YH D Representative flow plots of tetramer staining. E Combinatorial analysis of PD-1, TIM-3, LAG-3, and 2B4 inhibitory receptors revealed that YT—> AH had increased percent of CD8+ T cells that expressed 0 inhibitory receptors and decreased percent of CD8+ T cells that expressed at least 1 inhibitory receptor compared to AT—> AH. F Percentage of CD8+ T cells expressing granzyme B. G Percent functional virus-specific CD8+ T cells was calculated by dividing the percentage of granzyme B+ CD8+ T cells by the percentage of tet+ cells. H Boolean analysis of polyfunctionality of CD8+ T cells. Functional markers measured: granzyme B, IFNγ, TNF, perforin, and CD107a. *P < 0.05, **P < 0.005, ****P < 0.0001, one-way ANOVA

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Immunity & Ageing

ISSN: 1742-4933

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