Fig. 1

Deletion of Dsg3H1-Rag2^−/− T cells was disrupted in aged mice. a Outline of the adoptive transfer of Dsg3H1-Rag2^−/− T cells and WT CD4⁺ T cells to aged or young mice. b, c Flow cytometric (FCM) plots and quantitative summaries of aged and young mice after the transfer of 1 × 10⁶ CFSE-labeled Ly5.1⁺ Dsg3H1-Rag2^−/− T cells and 1 × 10⁶ Ly5.2⁺ WT T cells as an internal control (gated on CD4⁺). Proliferated CFSE^lowLy5.1⁺Dsg3H1-Rag2^−/− T cells and CFSE⁺Ly5.1⁻ co-transferred WT T cells were gated (red and black squares, respectively). Remaining Dsg3H1-Rag2^−/− T cell ratio was calculated as the proportion of CFSE^lowLy5.1⁺Dsg3H1-Rag2^−/− T cells (red squares) divided by that of CFSE⁺Ly5.1.⁻ WT T cells (black squares). The gating strategy was the same as that shown in Fig. S1b. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01 (unpaired t-test). Data were pooled from three independent experiments (n = 3–5 mice per group)