Fig. 5

HA-VLP and HA-VLP-Cyt vaccine-induced antibody inhibits hemagglutination of homologous but not heterologous viruses. HAI titers against the viruses (A/PR8, A/WSN, and A/Cal) were determined in sera from aged (A) and young (B) mice (n= 6/group) at 2 weeks after boost immunization with HA-VLP or HA-VLP-Cyt. 1 µg, 3 µg or 6 µg of each HA-VLP and HA-VLP-Cyt were administered. The statistical significances were determined using two-way ANOVA and indicated in *** P < 0.001. Error bars indicate mean ± SEM