Fig. 1
From: Metabolic phenotype of B cells from young and elderly HIV individuals
Gating strategies and a representative dot plot to show B cell subsets. PBMC (2 × 106/ml) from one young healthy individual were stained for 20 min at room temperature with anti-CD45, anti-CD19, anti-CD27 and anti-IgD fluorochrome-conjugated antibodies to evaluate naive (IgD + CD27-), IgM memory (IgD + CD27+), switched memory (swIg, IgD-CD27+), and DN (IgD-CD27-) B cells