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Fig. 2 | Immunity & Ageing

Fig. 2

From: Human MDSCs derived from the bone marrow maintain their functional ability but have a reduced frequency of induction in the elderly compared to pediatric donors

Fig. 2

Suppression of PBMCs proliferation by myeloid subsets sorted from BM or BM-MDSCs. CellTrace-labelled PBMCs activated with anti-CD3 and anti-CD28 were cultured in the presence of 1:1 ratio of the myeloid populations, unsorted (UNS), mature (CD11b+) or immature (CD11b−, BM-MDSCs). Immunosuppression of these populations was evaluated on activated T cells, (gated as CellTrace+/CD3+) normalized on the control without myeloid cells. (n = 20 independent experiments for pediatric patients, n = 20 for elderly patients) a Suppression was calculated by CellTrace profile assessed as the reduction of the proliferating CD3+ cells (those contained within generation two onward) in the co-culture condition as compared to T cells cultured alone, expressed as percentage. b Suppression was assessed by T cell number calculated by analyzing the absolute number of proliferating CD3+ cells by TruCount™ tubes. In both cases proliferation data were normalized assuming as 100% the proliferation rate of CD3+ cells cultured without myeloid cells

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Immunity & Ageing

ISSN: 1742-4933

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