Fig. 2

ARID3a expressing aged HSCs are not preferentially associated with markers of biological age. a Select HSCs from those presented in Fig. 1b were used to prepare genomic DNA and were assessed for telomere length by qPCR. Solid black points indicate aged samples, while open points indicate young samples. Square denote samples above the line of fit, triangles fall below the line and circles correspond with the line. Telomere lengths of HSCs from samples above and below (b), or on/near the trend line (c) are plotted against age. Telomere lengths are plotted against frequency (d), and total numbers of ARID3a + HSCs (e). f A representative flow cytometric plot of HSCs analyzed for ARID3a and H2A.xp139 is shown. g Frequencies of H2A.x⁺ HSCs from young (n = 5) and aged (n = 7) donors are shown. h Frequencies of H2A.x⁺ HSCs are plotted against age. i Frequencies of H2A.x⁺ HSCs are plotted against frequencies of ARID3a⁺ HSCs. Frequencies of H2A⁺ARID3a⁺ HSCs (j), and H2A⁺ARID3a⁻ HSCs (k) are plotted for young and old donors. Averages and standard error bars are indicated. Statistical significance was determined by Mann Whitney U test and Pearson’s Correlation. **, p = 0.0051