Fig. 3

Age-related expressions of total, M1, and M2 macrophages in kidney, liver and skeletal muscle. The Expression of total, M1 and M2 macrophages were determined by Immunohistochemistry and Immunoblotting. The level of Iba1 represented total macrophage expression in a kidney, b liver and c skeletal muscle. The level of iNOS represented M1 macrophage expression in d kidney, e liver and f skeletal kidney. The level of arginase 1 (Arg 1) represented M2 macrophage expression in d kidney, e liver and f skeletal kidney. Ratios in graphs are folds levels versus young mice. Scale bar = 100 um, *; p < 0.05 and **; p < 0.01 versus young mice