Fig. 3

Transcript levels of antimicrobial peptide encoding genes are differentially regulated in chico mutants following bacterial infection. Gene transcript levels for (a, b) Diptericin, (c, d) Cecropin-A1, and (e, f) Drosomycin in 7-10 day old Drosophila melanogaster chico mutants and yellow white (yw) background control flies at 3, 24 and 48 h after infection with non-pathogenic Escherichia coli (strain K12) or pathogenic Photorhabdus luminescens (strain TT01) bacteria. Gene transcript levels are shown as relative abundance of transcripts normalized to gene RpL32 and expressed as a ratio compared to flies injected with sterile PBS (negative control). Values represent the means from three biological replicates and error bars represent standard deviations. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05 (one way analysis of variance with a Tukey post hoc test, GraphPad Prism5 software)