Figure 5

Reduction in marginal zone B cell percentage in B cell-specific Ziz2 KO mice. (A) Splenocytes were analyzed by flow cytometry for T1 (Transition 1) and MZ B cells in CD23⁻ cells. T1: IgM⁺ CD21⁻. MZ: IgM⁺ CD21⁺. (B) The percentage of MZ B cells was lower in CD19^Cre/+; Ziz2^flox (Conditional KO) mice than in CD19^Cre/+ (Control) mice. (C) Splenocytes were analyzed by flow cytometry for T2 and mature (M) B cells in CD23⁺ cells. T2: IgM^high CD21⁺. M: IgM^low/middle CD21⁺. (D) The percentage of “M” was not altered in KO mice. **: P < 0.01 Between seven and ten mice (9–11 weeks old, male or female) per group from five independent experiments (from one to three mice per group per experiment) were used.