Figure 2

Early bone marrow B cell development was altered in KO mice. (A) Cell from BM were analyzed by flow cytometry for fractions “A-C” in CD43⁺ B220⁺ BM cells. Fraction “A” (Germline Pro-B): CD24⁻ BP1⁻. Fraction “B” (DJ-rearranged Pro-B): CD24⁺ BP1⁻. Fraction “C” (Pre-B early): CD24⁺ BP1⁺. (B) The percentage of fraction “A” was increased in KO mice. (C) Cells from BM were also analyzed by flow cytometry for fractions D-F in CD43⁻ B220⁺ BM cells. Fraction “D” (Pre-B late): IgM⁻ IgD⁻. Fraction “E” (Newly-formed B): IgM⁺ IgD⁻. Fraction “F” (Follicular-type recirculating B): IgM⁺ IgD⁺. (D) The percentage of fraction “F” was not altered in KO mice. 2KO: Ziz2 KO. 3KO: Ziz3 KO. *: P < 0.05 Three mice (8 weeks old, female) per group from three independent experiments (one mouse per group per experiment) were used.