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Figure 1 | Immunity & Ageing

Figure 1

From: Flow-cytometric assessment of cellular poly(ADP-ribosyl)ation capacity in peripheral blood lymphocytes

Figure 1

Frequency distributions (histograms) of MFI representing poly(ADP-ribosyl)ation levels in permeabilised Jurkat cells. (A) Untreated cells, no antibodies (Ab) added (negative control); (B) no NAD+ and no oligo in the reaction buffer; primary Ab, secondary Ab; (C) no NAD+, but oligo in the reaction buffer; primary Ab, secondary Ab; (D) NAD+, but no oligo in the reaction buffer; primary Ab, secondary Ab; (E) NAD+ and oligo in the reaction buffer; primary Ab, secondary Ab. Note that a shift to the right (FL-1; x-axis) indicates increased levels of poly(ADP-ribosyl)ation. Numerical values next to the histograms represent the mean fluorescence intensity (MFI). M1, intensity range used to determine mean of FL1-H.

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Immunity & Ageing

ISSN: 1742-4933

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